It's common to know peptide libraries are in ever increasing demand due to the expanding interest in peptide production and vaccine development. In fact, peptide library can be synthesized on a solid phase that could be made as flat surface or beads. However, when it comes to the applicable peptide library design, it’s a little different.
There are some differences in varying substances. Peptide library design, seen as an available biological research tool, is able to provide a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. In addition, it is widely used in related biological research for screening large numbers of peptides in the needs for the few but critical bioactive peptides. Modern technical and applications can be of great help for indicating advice on library design and experimental analysis, such as peptide target application, full-length protein sequence, and so on. Generating a related list of various peptides is necessary for the library design.
Clearly, it's common to know assistance with experimental trail is available for applications such as cell culture, intracellular cytokine staining and flow cytometry. In fact, peptide library design technology can be used for a number of different types of library, including overlapping peptide library, alanine scanning library, random library, etc. Taking the overlapping peptide library as an instance, it is most commonly used for linear, continuous epitope mapping. The aim is to generate a library of overlapping peptide sequences of specific length and specific offset, enabling to cover the entire native protein sequence. Based on the fact, how to choose the appropriate peptide length and offset number may be some difficult. But with the support of modern peptide library, it’s possible to use obtained data from the experiment to gain larger success.
In addition, to be more specific, choosing appropriate peptide length and offset number is illustrated with two extreme situations, which might requires enough attention. In terms of alanine scanning library, it is systematically substituted into each amino acid position in the identified epitope. Therefore, useful strategy like peptide design library is indispensable for modern drug design.
